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. 2021 Jan 19;108(2):309–323. doi: 10.1016/j.ajhg.2021.01.002

Figure 5.

Figure 5

Abnormal CFAP65 location in the spermatozoa from men harboring hemizygous CFAP47 variants

Sperm cells were stained with anti-CFAP65 (red) and anti-α-tubulin (green) antibodies. DNA was counterstained with DAPI as a marker of the cell nucleus. CFAP65 staining was observed mainly at the equatorial segment of sperm head and the base of flagella in control spermatozoa, but the signal was only diffusely clustered in the acrosome of spermatozoa from men harboring hemizygous CFAP47 variants. Scale bars: 5 μm.