Figure 8.

Damaged fertilization capability by deficiency of mouse CFAP47 could be rescued by ICSI

(A) Representative two-cell embryos and blastocysts from IVF in mice. The rates of both two-cell embryos and blastocysts were significantly lower in the group using the spermatozoa from Cfap47-mutated (Cfap47^‒/Y) male mice than those in the wild-type (Cfap47⁺/Y) group. Both groups consisted of four male mice. A total of 716 mouse embryos were counted. Data are represented as means ± standard error of measurement; ^∗∗∗p < 0.001. Scale bars: 200 μm.

(B) Representative two-cell embryos and blastocysts from ICSI in mice. The rates of two-cell embryos and blastocysts were counted after sperm heads were injected into oocytes that were collected from superovulated wild-type female mice. Both the Cfap47⁺/Y and Cfap47^‒/Y groups consisted of three male mice. A total of 367 mouse embryos were counted. Data are represented as means ± standard error of measurement. n.s., not significant. Scale bars: 200 μm.