Figure 7.
Transcriptional effects of SALL4 zinc-finger cluster mutations during neuronal differentiation
(A) Diagram of the RNA-seq time-course experiment comparing the differentiation potential of WT and Sall4 mutant ESCs.
(B) Differential gene expression analysis between WT and Sall4 mutant cell lines during neuronal differentiation at days 2 and 5 (adjusted p value < 0.05). Additional WT replicates were used as a control (WT versus WT).
(C) Scatterplot showing the relative expression levels of genes deregulated in differentiating S4KO cells (see B, gray bars) correlating with their expression in ZFC4mut cells at days 2 and 5 of differentiation.
(D) Relative expression levels (log2 fold change versus WT) of genes associated with the GO term “positive regulation of neuron differentiation” (GO:0045666) in Sall4 mutant cell lines at days 2 and 5 of differentiation. Additional WT replicates were used as a control (WT versus WT). Stars indicate statistical significance calculated from bootstrapped two-sided t tests (p value < 0.05).