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. 2021 Feb 18;184(4):1110–1121.e16. doi: 10.1016/j.cell.2021.01.033

Figure 2.

Figure 2

Design and structural characterization of signpost origami

(A) Four views of a ribbon (backbone) and plank (base pairs) representation of the signpost origami design. Scale bar, 20 nm.

(B) Negative-stain EM projection image (top; scale bar, 100 nm) and selected particles in different orientations (bottom; scale bar, 25 nm) of the folded signpost origami.

(C) CryoEM projection image (top; scale bar, 100 nm) and selected particles (bottom; scale bar, 50 nm) show significant contrast of the folded signpost origami in a background of cell lysate.

(D) Four views of a 3D structure of the signpost origami obtained from cryoET and sub-volume averaging. This experimentally determined structure closely matches the designed structure in (A). The DNA design of this signpost origami is given in Figure S2. Scale bar, 10 nm.