Figure 5.

SPOTs binding to YFP-tagged Env in the envelope of murine leukemia virus (MLV)

Computed slices through the tomogram reconstructed from the tilt series shown in Figure S5G. The slice position in the reconstructed volume increases in a clockwise manner and is recorded in each panel as text and by the colored frame indicated in the central schematic diagram, where the virus is represented by the orange sphere. In the first panel (z = 0 nm), the inside of the viral membrane and approximate distal surface of the viral glycoproteins tagged with YFP are indicated by the solid orange and dashed yellow circles, respectively. A single SPOT is highlighted in blue. Tomogram slices are ∼0.5 nm thick. Scale bar, 100 nm.