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. 2021 Feb 19;12:1159. doi: 10.1038/s41467-021-21372-4

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative (>6 embryos) live-imaged neuroepithelium before and after annular laser ablation. Ablations were made in regions including (with EGFP) or excluding (no EGFP) Vangl2-deleted cells. b Particle image velocimetry (arrows superimposed on grey cell borders) illustrating apical retraction of a cluster of cells within the ablated annulus. c Quantification of the retraction (% change in area) of cell clusters within the ablated annulus. Dots represent a cluster in an individual embryo; each embryo was only ablated once. Control n = 20, EGFP+ n = 10, No EGFP n = 6 embryos. d Schematic illustration of cell retraction due to immediate constriction when released from connections to surrounding cells. e, f Representative live-imaged neuroepithelium from control (No Cre, 20 embryos) and Cre;Fl/− (10 embryos). A cell is traced before and after ablation in the No Cre control. The # and * correspond to the cells shown in f, illustrating retraction. g Quantification of the retraction (% change in area) of individual cells within the ablated annulus. Ablations included 2–3 Vangl2-deleted (EGFP) and Vangl2-replete (tdTom) neighbours of the deleted cells. Retractions of cells of the same type were averaged in each cluster, n = 10 embryos per genotype. Scale bars = 20 µm. p values from ANOVA with post-hoc Bonferroni.