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. 2021 Feb 19;12(2):201. doi: 10.1038/s41419-021-03489-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Analysis of lncRNAs which were differentially expressed and related to KIRC survival in TCGA, using circlncRNAnet and OncoLnc database, respectively. b Venn diagram presenting the lncRNAs that were upregulated (1808 lncRNAs), related to survival (1558 lncRNAs), or both (14 lncRNAs) in KIRC. c The 14 lncRNAs which were upregulated and related to a shorter survival in KIRC. d qRT-PCR analysis of CDKN2B-AS1 expression in 42 paired KIRC tissues (tumor) and adjacent normal tissues (adjacent); β-actin was used as the internal control. e–h CDKN2B-AS1 expression was analyzed in KIRC patients regarding tumor size (e), histologic grade (f), TNM stage (g), and metastasis status (h), using RNA-seq data in TCGA. Error bars represent the SEM. i, j Kaplan–Meier analysis showed the overall survival and disease-free survival in KIRC patients based on the expression of CDKN2B-AS1 in TCGA dataset, the high and low expression groups were divided by the median of CDKN2B-AS1 levels. P value calculated by paired-sample t test (d), independent sample t test (e, h), one-way ANOVA (f, g), or log-rank test (i, j).