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. 2021 Jan 6;120(3):476–488. doi: 10.1016/j.bpj.2020.12.019

Figure 1.

Figure 1

Effect of Hsp70 on aggregation of IAPP. (A–C) Kinetics of aggregation of 20 μM IAPP in presence of substoichiometric concentrations of human Hsc70 (A), E. coli DnaK (B), and MTB-Hsp70 (C) is shown. The squares, diamonds, triangles, and circles represent molar ratios of 0:1, 1:400, 1:200, and 1:100 of Hsp70/IAPP, respectively. (D) Fold increase of the “lag” phase of aggregation of 20 μM IAPP by 80 nM Hsp70 under various solution conditions is shown. Concentration of DnaJ used is 80 nM and of ATP and ADP is 2 mM is shown. The solutions are prepared in 10 mM phosphate buffer at pH 7.4, containing 150 mM NaCl, 1 mM EDTA, and 2 mM sodium azide. The experiments are performed at 37°C with continuous stirring. In the ATP/ADP-dependent experiments, NaCl and EDTA are substituted with 100 mM KCl and 5 mM MgCl2. (E and F) AFM images of the 20 μM IAPP incubated for 48 h in absence or presence of 0.5 μM DnaK are shown. All the experiments have been performed at least twice. To see this figure in color, go online.