Figure 2.

Effect of Hsp70 on different stages of IAPP aggregation. 160 nM Hsc70 (A) or 200 nM DnaK (B) is added to a 20-μM IAPP solution at different stages of aggregation (viz, at t = 0 (circles)), in the middle of “lag”’ phase (t = 1 h) (triangles), in the late lag phase (t ∼ 2 h) (diamonds), and in the growth phase (t ∼ 2.4 h) (squares). Arrows indicate the time points when Hsp70 was added. Clearly, the effect of Hsp70 is highest if added at t = 0. Solutions are prepared in PBS buffer containing 1 mM EDTA and 2 mM sodium azide at pH 7.4 and incubated at 37°C with continuous stirring. These experiments have been performed twice. To see this figure in color, go online.