Figure 6.

Characterization of IAPP-DnaK complexes after fractionation by size exclusion chromatography (SEC) or field flow fractionation (FFF). (A) SEC-MALS and (C) FFF-MALS of 10 μM DnaK in absence and presence of 20 μM IAPP are shown. The solid lines indicate refractive index (RI), which is a measure of concentration, and empty symbols represent molecular weight (MW) measured by MALS. (B) R_h measured using FCS of TMR-IAPP, TMR-DnaK, and TMR-IAPP + DnaK after purification using SEC or FFF is shown. Clearly, R_h of TMR-IAPP + DnaK is similar to TMR-DnaK, indicating binding of TMR-IAPP to DnaK. The solutions are prepared in PBS buffer containing 1 mM EDTA and 1 mM sodium azide at pH 7.4 and incubated at RT for O/N. These experiments have been performed four times. To see this figure in color, go online.