Table 1.

Calculated metal occupancies of COG0523 proteins in an idealised cell^a.

Metal	MgIIGTP-CobW Eq. 2b	MgIIGTP-CobW Eq. 4c	MgIIGTP-YeiR Eq. 4c	MgIIGTPγS-YjiA Eq. 4c
MnII d			<0.8%	<1.8%
FeII d	<4.6%	<0.1%	<3.0%	< 3.4%
CoII	98.8%	91.9%	10.3%	2.0%
ZnII	86.2%	6.9%	24.4%	22.6%
NiII	0.1%	0.0%	0.0%	0.0%
CuI	0.5%	0.0%	0.2%	0.1%
Total	190.3%	98.9%	38.6%	29.8%

^aBased on metal availabilities in Salmonella under idealised conditions (ref. ⁹).

^bDoes not account for competition between different metals for the same high-affinity site in Mg^IIGTP-CobW.

^cTakes into account competition between multiple intracellular metals for the same site in each protein.

^dWhere only limiting metal–protein affinities (strongest K_D limit) were determined, calculated occupancy corresponds to a maximum value (denoted by <).