Effect of varying the amplitude of the second phase of the 2-ns bipolar pulse on Ca2+ responses. (a) Response of the cells to a 2-ns unipolar pulse (n = 18). Results are plotted as the averaged fluorescence traces ± SEM for unexposed cells (gray traces; n = 9) and pulse-exposed cells (blue traces). (b–f) Averaged fluorescence traces ± SEM for cells exposed to 2-ns bipolar pulses for second phase amplitude ratios of 0.5 (n = 16), 1.0 (n = 9), 1.5 (n = 23), 1.8 (n = 8), and 2.0 (n = 24), respectively. A representative pulse waveform is provided in each plot, and the arrow indicates the time when the pulse was applied. To see this figure in color, go online.