Figure 1.

Detecting yolk-granules in a C. elegans embryo in DIC. (a) The yolk granules of a C. elegans embryo in the one-celled stage in DIC microscopy from the mid-plane are segmented using a novel scaling of Gaussian (SoG) method combined with automated thresholding. Scale bar, 5 μm. (b) The workflow is described using a representative region of interest (ROI) of the embryo image (box with dashed blue line from (a)); shown is a filtered image by convolving the image with an SoG filter (parameters: k_size = 9 and σ = 1.25), resulting in enhanced contrast; shown is a segmented image obtained by thresholding by a modified Otsu’s method; and centroids of detected objects (asterisk) are overlaid on the original image. To see this figure in color, go online.