Six6os1ΔC/ΔC male mice displayed meiotic arrest and incomplete synapsis between homologous chromosomes
(A) The progression of meiotic prophase I was analyzed by immunostaining surface-spread spermatocytes from wild-type and Six6os1ΔC/ΔC mice with antibodies against SYCP3 (red) and γH2AX (green). Representative images of leptotene, zygotene, pachytene, and diplotene spermatocytes from wild-type mice are shown. The meiosis of Six6os1ΔC/ΔC mice was arrested at the pachytene-like stage.
(B and C) Immunofluorescence staining of surface-spread spermatocytes from wild-type and Six6os1ΔC/ΔC mice with antibodies against SYCP3 (red) and SYCP1 (B, green) or SYCE1 (C, green).
(D) Representative images of surface-spread spermatocytes from wild-type and Six6os1ΔC/ΔC mice stained with antibodies against SYCP3 (red) and SIX6OS1-N (green). The white arrows in (A) indicate the sex body, and the arrows in (B), (C), and (D) indicate sex chromosomes. Scale bars denote 10 μm.