Figure 9.

Quantification of GluRIIA levels at 1b NMJs. A–F, Representative GluRIIA immunostaining from m6 (A, B), m12 (C, D), and m4 (E, F) in control (A8>GFP) and 1s-ablated animals (A8>GFP,hid,rpr) labeled with GFP (green), GluRIIA (red), and DLG (blue). A′–F′, 2.5× zoomed 1b boutons corresponding to dashed regions in A–F showing GluRIIA staining. G, Quantification of normalized total GluRIIA intensity of m6 (t_(18.38) = 3.812, p = 0.0012, unpaired t test with Welch's correction), m12 (t₍₂₈₎ = 1.337, p = 0.1921, unpaired t test), and m4 (t₍₂₆₎ = 2.049, p = 0.0507, unpaired t test). Images and graphs are color coded as indicated in the color key. H, Quantification of GluRIIA/DLG sum intensity ratio (density) of m6 (t₍₂₉₎ = 1.108, p = 0.2771, unpaired t test), m12 (t₍₂₈₎ = 0.8544, p = 0.4002, unpaired t test), and m4 (t₍₂₆₎ = 0.2679, p = 0.7909, unpaired t test). Error bars indicate ±SEM. **p < 0.01, ns - not significant. n values (NMJs/larva) are 16/8, 15/8, 14/7, 16/8, 15/8, and 13/7, respectively.