Figure 10.

TB1-associated deficits in NOR 30 dpi were microglia dependent. A, Adult C57BL/6 mice were provided diets formulated with either vehicle (Veh) or PLX5622 (PLX) for 14 d. Next, mice were uninjured (control) or were subjected to mFPI (TB1). Mice were maintained on vehicle or PLX diet for the duration of the experiment. In the first study (n = 6), mice were subjected to the accelerating Rotarod and wire-hang test at multiple time points (1, 7, 14, 21, and 28 dpi). In the second study (n = 10), mice were treated as above and NOL and recognition was assessed 28–31 dpi. B, Latency to fall from the accelerating Rotarod and (C) latency to escape from horizontal bar were determined. In a separate cohort, mice were used in the object location and recognition tests. At 30 dpi, (D) total time spent exploring, (E) percent of time spent investigating the novel location, and (F) discrimination index for the novel location were determined. At 31 dpi, (G) total time spent exploring, (H) percent of time spent investigating the novel object, and (I) discrimination index for the novel object were determined. Graphs represent mean ± SEM. Means with * are significantly different from vehicle controls (p < 0.05).