Figure 8.

Increased dendritic remodeling 7 dpi was dependent on microglia. A, Adult Thy-1 GFP⁺ mice were provided diets formulated with either vehicle (Veh) or a CSF1R antagonist (PLX5622) for 14 d. Next, mice were uninjured (Con) or were subjected to mFPI (TB1). Mice were maintained on vehicle or PLX diet for the duration of the experiment. At 7 dpi, mice were perfused, fixed, and brains were cryosectioned and mounted on slides for microscopy analysis of dendritic density and morphology (n = 6). B, Representative images of cortical neuron reconstructions (generated in Imaris) at 7 dpi from each treatment group. C, Sholl analysis of intersections by radius in the cortex. D, Sholl intersections area under the curve. E, Images of Thy-1 GFP ⁺ dendrites 7 dpi. F, Cortical dendritic spine number. G, Dendritic spine density classified by mature mushroom or immature stubby spines. Bars represent mean ± SEM. Means with * are significantly different from Veh-Con group (p < 0.05).