Figure 9.

TB1-associated deficits in neuronal connectivity 30 dpi were microglia dependent. Adult C57BL/6 mice were provided diets formulated with either vehicle (Veh) or PLX5622 (PLX) for 14 d. Next, mice were uninjured (control) or were subjected to mFPI (TB1). Mice were maintained on vehicle or PLX diet for the duration of the experiment. A, At 7 and 30 dpi, mice were killed and CAP was determined from ex vivo preparation of the corpus callosum (n = 6). Representative N1 and N2 tracings of CAP from control and TB1 mice 7 dpi are shown. Graphs reflect average recording amplitude across range of stimulus intensities for (B) N1, (C) N2, and (D) N2/N1 ratio. Recorded values for maximum stimulus intensity (2 mA) are shown for (E) N2/N1 ratio. Graphs reflect averages ± SEM. Means with * are significantly different from Veh-Con group (p < 0.05). Means with # tend to be significantly different (p = 0.06) from Veh-Con group.