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. 2021 Feb 19;12(2):199. doi: 10.1038/s41419-021-03487-0

Fig. 7. SNEP1 induces vismodegib-resistance in colorectal cancer cells.

Fig. 7

A–D HCT-116 or Caco2 cells stably expressing LV-SNEP1 were seeded in a 6-well plate for 14 days, and treated with 2.5 μM vismodegib. The cells were then stained with 0.5% crystal violet (w/v) (A and C). Quantitative analysis was performed using Image J software (B and D). The bar graph displays the mean ± SD, n = 3, **p < 0.01, N.S., not significance. E, F Relative cell viability and IC50 of vismodegib in control or SNEP1-overexpressing HCT-116 cells (E), Caco2 cells (F). Data represent mean ± SD of three separate experiments. G Schematic showing how SNEP1 and LNX1 work together to degrade SuFu via a ubiquitin-dependent mechanism to promote CRC development by activating the Hh pathway.