Table 1.

Summary of HDX-MS experimental parameters⁵³.

Data set	Control	IGF-1	VHH-IR5
HDX reaction details	PBS, pD = 7.0, 25 °C
HDX time course (min)	1, 3, 10, 60
# of Peptides found across all samples	133
Sequence coverage	47%
Average peptide length/redundancy	11.7/1.7
Replicates (biological or technical)	3 (technical)
Repeatability (average standard deviation, %)	1.6	1.5	1.4
Significant differences in HDX	Two-state student T-Test performed at each time point (> 2 SD, p value 0.05)

*All HDX-MS experiments were carried out with recombinant and SEC-purified eIGF1R (Fig. S3).