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. 2021 Feb 19;4:233. doi: 10.1038/s42003-021-01758-2

Fig. 5. In vitro stability and viability evaluation and in vivo compatibility studies.

Fig. 5

a In vitro degradation study of the engineered fiber scaffold. i PCL, PCLMA and PCLMA-UV in a lipase system, PCLMA:GelMA (70:30) and PCLMA:GelMA (70:30)-UV in ii, lipase and in iii, collagenase Type II systems, respectively. Values are mean ± SD, N = 3. b Cell viability experiments on the various fibers using NIH/3T3 fibroblast cells after 7 days. Values are mean ± SD, N = 3. c Proliferation and spreading images of the fibers on NIH/3T3 fibroblast cells after 7 days. Histological evaluation after subcutaneous implantation in the dorsal region of rats for five days. d, i Capsule thickness ii Tissue response of the capsule surround implants iii Tissue response directly adjacent to the implant surface and iv blood vessel of the subcutaneous implants using the histological grading scale (Supplementary Table 2). Error bars represents mean ± SD of the mean, N = 3; × p < 0.05 compared with PCL and GelMA; *p < 0.05 compared with PCL; #p < 0.05 compared with PCL and GelMA. e The photomicrographs of subcutaneous implants of i PCL, ii GelMA, iii PCLMA-UV, iv GelMA-UV and v PCLMA:GelMA (70:30)-UV. ●Nanofiber, ←Blood vessel and ▲cell inflammatory and @panniculus carnosus muscle. Hematoxylin and Eosin staining (H & E staining), scale bar = 50 µm.