Figure 7.

8-isoprostane urine excretion per 24 h (A), renal TBARS (B), nitrate and nitrite (NOx) urine excretion (C) and renal endothelial nitric oxide synthase (eNOS) to β-actin ratios expressed as a percent change from the average of the control HanSD group (D) in control (CON), untreated heart failure (HF), ACE inhibitor-treated (ACEi) and AT₁ receptor blocker-treated (ARB) HanSD and TGR rats 20 weeks after sham or ACF operation (5 weeks in HF TGR rats due to high mortality). Shown in (E) are representative western blot images of renal eNOS and β-actin. These images were cropped to improve clarity. All samples were derived from the same experiment and were processed in parallel. Original unprocessed blots can be found in Supplementary Fig. 1. Data presented as mean ± SD (n = 6–9 in each group). ^#P < 0.05 compared to HanSD rats.