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. 2021 Feb 19;11:4280. doi: 10.1038/s41598-021-83339-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Percentages of fluorescence intensities of thiamphenicol (zoomed to 80–100%) in the extracellular environment measured during the incubation of WT (grey boxes) and P23 (blue boxes) strains. The box-and-whisker plot shows the medians (“center” lines), 25th (lower section) and 75th (upper section) percentiles (interquartile range; box edges) and the span of the data for each sample (whiskers). ANOVA and Tukey’s post-hoc tests were performed to determine differences between the two strains (6 min, n = 17; 9 min, n = 18; 12 min, n = 14; 15 min, n = 18). ***p < 0.001; **p < 0.01; *p < 0.05. Data standardization was performed by the Shapiro–Wilk test, and homogeneity of variances was checked by the Fligner-Killeen test. Ns not significant. The significant difference between the two strains for the entire incubation kinetics was extremely significant p < 0.001 (ANOVA, n = 67, Cohen’s f = 0.51). Four independent experiments were performed (with technical triplicate).