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. 2021 Feb 19;12(2):203. doi: 10.1038/s41419-021-03492-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Immunoblot analysis of p-Src, p-ERK1/2, and MMP3 in U87-C1, U87-GBP5, U251-C1, and U251-GBP5 cells. GAPDH was used as loading control. B Immunoblot analysis of p-Src, p-ERK1/2, and MMP3 in SNB19shGFP and SNB19shGBP5 cells. GAPDH was used as loading control. C Immunoblot analysis of MMP3 in U87-GBP5 cells with or without MMP3 silencing with siRNA. D Effect of MMP3 knockdown on the GBP5-driven cell proliferation. ***p < 0.001. n = 3. E Effect of MMP3 knockdown on the GBP5-driven cell invasiveness by the matrigel transwell assay. Invasion index = OD570 of invaded cells in treatment groups/OD570 of invaded cells in control groups. **p < 0.01. NC = not statistically significant. n = 3.