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. 2021 Feb 19;12(2):203. doi: 10.1038/s41419-021-03492-3

Fig. 4. Src/ERK regulates GBP5-promoted MMP3 expression, cell proliferation and invasion.

Fig. 4

A Immunoblot analysis of p-Src, p-ERK1/2, and MMP3 in U87-C1, U87-GBP5, U251-C1, and U251-GBP5 cells. GAPDH was used as loading control. GBP5-expressing U87 and U251 cells were separately treated with pharmacological inhibitors of Src (PP2), ERK1/2 (U0126), and dimethyl sulfoxide (DMSO) vehicle for 8 h. B Effect of the blockade of Src or ERK1/2 with the inhibitors on the proliferation of U87 and U251 cells. **p < 0.01; ***p < 0.001. n = 3. C Effect of the blockade of Src or ERK1/2 with the inhibitors on the invasion of U87 and U251 cells. Invasion index = OD570 of invaded cells in treatment groups/OD570 of invaded cells in control groups. **p < 0.01; ***p < 0.001. NC = not statistically significant. n = 3.