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. 2021 Feb 19;12:1160. doi: 10.1038/s41467-021-21429-4

Fig. 1. Central brain PDF-Tri neurons are transient and eliminated following development.

Fig. 1

a Central brain PDF-Tri neurons in a newly eclosed adult Drosophila co-labeled for PDF-Gal4-driven UAS-mCD8::GFP (PDF > GFP, green, left), anti-PDF (magenta, middle), and merged (right). The dorsal (D)–ventral (V) orientation is indicated, with the PDF-Tri neuron soma (asterisk) dorsal to the subesophageal zone (SEZ) and axons projecting via the median bundle (arrows). Scale bar: 10 μm. b PDF-Tri neuron clearance shown in days post eclosion (DPE) at 25 °C. Low-magnification whole-brain images are co-labeled for both membrane marker PDF > GFP (green) and anti-PDF (magenta), as above in panel a, with the developmentally transient central PDF-Tri neurons and adult-persisting ventral lateral neurons (LNVs). Scale bar: 50 μm. c High-magnification images of the PDF-Tri neurons (boxed central brain region in panel b). The timing sequence shows progressive elimination of the PDF-Tri neurons over the first 5 days of post-eclosion development. Scale bar: 12 μm. Images are representative of three independent experiments.