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. 2021 Feb 19;12:1160. doi: 10.1038/s41467-021-21429-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Brains expressing membrane-tethered mCD8::GFP in glial classes: (1) astrocyte-like glia (ALG, R86E01-Gal4 > mCD8::GFP, left), (2) ensheathing glia (EG, R56F03-Gal4 > mCD8::GFP, middle), and (3) cortex glia (CG, R54H02-Gal4 > mCD8::GFP, right). Images are representative of three independent experiments. b 5 DPE brains labeled with anti-PDF in ALG driver control (R86E01-Gal4/+) and expressing draper-RNAi (R86E0-Gal4 > draper-RNAi). Right: Graphs show anti-PDF area (left) and puncta (right). Area: Two-sided t test, p = 0.0622, 52.55 ± 10.6 n = 13 control, 136.3 ± 35.71 n = 18 draper-RNAi. Puncta: Two-sided t test, p = 0.214, 11.29 ± 2.471 n = 14 control, 17.78 ± 4.068 n = 18 draper-RNAi. c EG driver control (R56F03-Gal4/+) and draper-RNAi (R56F03-Gal4 > draper-RNAi). PDF-Tri neurons (arrows) absent in controls, but persist with Draper activity blocked. Right: Graphs show area (left) and puncta (right). Area: Two-sided t test, p = 0.0003, 33.65 ± 10.14 n = 16 control, 230.8 ± 48.787 n = 15 draper-RNAi; Puncta: Two-sided Mann–Whitney, p = 0.0003, 7.176 ± 1.867 n = 17 control, 26.38 ± 3.936 n = 16 draper-RNAi. d CG driver control (R54H02-Gal4/+) and draper-RNAi (R54H02-Gal4 > draper-RNAi). Right: Graphs show area (left) and puncta (right). Area: Two-sided t test, p = 0.0008, 65.11 ± 23.33 n = 12 control, 199 ± 25.09 n = 12 draper-RNAi; Puncta: Two-sided Mann–Whitney, p = 0.0039, 10.25 ± 3.148 n = 12 control, 22.29 ± 2.653 n = 14 draper-RNAi. Scatter plot graphs: mean ± SEM. Sample size is n = number of animals. Significance: p > 0.05 (not significant, N.S.), p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****). Scale bar: 50 μm. Source data for this figure are provided in Source Data file.