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. 2021 Feb 19;12:1160. doi: 10.1038/s41467-021-21429-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative anti-Draper Western blot of whole-brain lysates (2 brains per lane, 0 DPE) for control (w¹¹¹⁸) and dfmr1 null (w¹¹¹⁸; dfmr1^50M). All bands labeled on the left: Draper-I (Drpr-I, top), Draper-II/III (Drpr-II/III, middle), and α-tubulin (bottom). Molecular weights listed on the right (~kDa). Right: Graphs showing Draper-I (left) and Draper-II/III (right) levels normalized to α-tubulin in both genotypes. Drpr-I: two-sided t test, p = 0.0001, 1.000 ± 0.03750 n = 9 control, 0.7156 ± 0.04221 n = 9 dfmr1. Drpr-II/III: two-sided t test, p = 0.9076, 1.000 ± 0.06112 n = 9 control, 0.9908 ± 0.04875 n = 9 dfmr1. Sample size is n = number of independent protein extractions (two brains/extraction). b Representative Western blots for glial engulfment proteins in control and dfmr1 null. Labels listed left, and weights on the right. Images are representative of two independent experiments (see Supplemental Fig. 5). c Whole brains (5 DPE) labeled with anti-PDF in transgenic glial driver control (repo-Gal4/+), dfmr1 null with glial driver (dfmr1^50M, repo-Gal4/dfmr1^50M), and driving UAS-Draper-I (UAS-Drpr-I/+; dfmr1^50M, repo-Gal4/dfmr1^50M). Scale bar: 50 μm. Right: Quantification of anti-PDF area (left) and puncta (right). ANOVA followed by Tukey’s multiple comparison test. Area: p < 0.0001, 14.1 ± 6.406 n = 17 control, 560.6 ± 89.03 n = 22 dfmr1; p = 0.152, 14.1 ± 6.406 n = 17 control, 187.4 ± 37.5 n = 17 UAS-Drpr-I; p = 0.0002, 560.6 ± 89.03 n = 22 dfmr1, 187.4 ± 37.5 n = 17 UAS-Drpr-I. Puncta: 3.0 ± 5.347 p = 18 control, 44.95 ± 5.211 n = 22 dfmr1; p = 0.0006, 3.0 ± 5.347 n = 18 control, 28.67 ± 4.981 n = 21 UAS-Drpr-I; p = 0.00272, 44.95 ± 5.211 n = 22 dfmr1, 28.67 ± 4.981 n = 21 UAS-Drpr-I. Sample size is n = number of animals. Scatter plots show mean ± SEM. Significance: p > 0.05 (not significant, N.S.), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****). Source data for this figure are provided in Source Data file.