Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 19;12:1160. doi: 10.1038/s41467-021-21429-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a Whole brains (5 DPE) labeled with anti-PDF in the control (w; InR^wildtype(HR), right) and the insulin receptor (InR) mutant (w; InR^E19(HR), left). The PDF+ adult-persistent ventral lateral neurons (LN_vs) within the lateral brain optic lobes serve as valuable internal controls for both brain labeling and orientation. The developmentally transient PDF-Tri neurons along the central brain midline are absent in the matched genetic background controls, but persist when the InR signaling function is eliminated (arrows). Scale bar: 50 μm. b Quantification of anti-PDF area (left) and PDF+ puncta (right) in the two genotypes. Area: two-sided t test, p = 0.0002, 39.84 ± 10.94 n = 19 control, 168.4 ± 28.86 n = 19 InR^E19(HR). Puncta: two-sided Mann–Whitney, p < 0.0001, 6.563 ± 1.891 n = 16 control, 41.00 ± 6.841 n = 19 InR^E19(HR). Scatter plot graphs show mean ± SEM. Sample size is n = number of animals. Significance: p < 0.001 (***) and p < 0.0001 (****). Source data for this figure are provided in Source Data file.