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. 2021 Feb 19;12:1160. doi: 10.1038/s41467-021-21429-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Central brains (2 DPE) co-labeled for glial mCD8::GFP (left), anti-phosphorylated insulin receptor (InR^P, middle), and merge (right) in the control (UAS-mCD8::GFP/+; repo-Gal4/+) and dfmr1 null (UAS-mCD8::GFP/+; dfmr1^50M, repo-Gal4/dfmr1^50M). White outline indicates glial area used for InR^P measurements; arrows indicate glial cell bodies. Range indicator bar for InR^P intensity level below. Scale bar: 10 μm. b Whole brains (5 DPE) anti-PDF labeled in glial driver control (repo-Gal4/+), dfmr1 null with driver (dfmr1^50M, repo-Gal4/dfmr1^50M), and InRCA targeted to glia (UAS-InR^del/+; dfmr1^50M, repo-Gal4/dfmr1^50M). PDF-Tri neurons (arrows) in dfmr1 null. Scale bar: 50 μm. c Quantification of normalized InR^P levels from panel a. Two-sided t test, p = 0.0054, 1.00 ± 0.0815 n = 12 control, 0.694 ± 0.0521 n = 11 dfmr1. d Quantification of the PDF-Tri area from panel b. ANOVA followed by Tukey’s multiple comparison test, p = 0.0011, 18.18 ± 9.959 n = 7 control, 952.3 ± 211.9 n = 10 dfmr1; p = 0.3183, 18.18 ± 9.858 n = 7 control, 352.8 ± 105.9 n = 10 InRCA; =0.0197, 352.8 ± 105.9 n = 10 InRCA, 952.3 ± 211.9 n = 10 dfmr1. e Quantification of PDF+ puncta from panel b. ANOVA followed by Tukey’s multiple comparison test, p < 0.0001, 3.286 ± 1.686 n = 7 control, 62.6 ± 7.524 n = 10 dfmr1; p = 0.0118, 3.286 ± 1.686 n = 7 control, 32.9 ± 6.28 n = 10 InRCA; p = 0.0054, 32.9 ± 6.28 n = 10 InRCA, 62.6 ± 7.524 n = 10 dfmr1. Scatter plot graphs show mean ± SEM. Sample sized is n = number of animals. Significance shown for p > 0.05 (not significant, N.S.), p < 0.05 (*), p < 0.01 (**), and p < 0.0001 (****). Source data for this figure are provided in Source Data file.