Radius of gyration and occurrence of the distances measured between PNA nucleobases (A) and residual root mean-square fluctuations (B) of the vitamin B12-(CH2)12-PNA conjugate residues during permeation through BtuB. Labels are defined as follows: L12, linker L12; C, A, T, and G, PNA monomers; K, lysine. For the radius of gyration, the median and lower-upper extremes range are provided. To see this figure in color, go online.