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. 2020 Jun 30;41(11):1529–1542. doi: 10.1093/carcin/bgaa064

Figure 4.

Figure 4.

Estrogen treatment in male LR/Stat3Δ/Δ mice reprogrammed lung tumor microenvironment to an anti-tumor phenotype through suppressing NF-κB activity. (a) Total inflammatory cell and lineage-specific leukocyte numbers from BALF (n = 6, 7). (b) Relative mRNA expression of Retnla1, Arg1, Mrc1 and Ido1 mRNA in BALF inflammatory cells, normalized by Cd45 expression. (c) Relative mRNA expression of Ccl2, Cxcl1, Il6, Il17a, Tnf, Tbx21, Il10, Foxp3, Gzmb, Ifng, Arg1, Cd274 and Tgfb1 in the whole lung, normalized by Cd45 expression. (d) Relative mRNA expression of IκBα in the whole lung normalized to β-actin. (e) Fold change of p65 binding activity represented by fold change of OD value. (f) Western blot analysis of IκBα and β-actin protein levels in whole lung tissue. (g) Quantification of (f) relative density of IκBα/β-actin. Data represent mean ± SEM; ****P < 0.0001, **P < 0.01, *P < 0.05, experimental replicate #3.