Figure 6.
Lung epithelial-specific deletion of IKKβ reprogrammed lung tumor microenvironment in LR/Stat3Δ/Δ male mice through suppressing NF-κB activity. (a) Total inflammatory cell and lineage-specific leukocyte numbers from BALF (n = 6, 7). (b) Relative mRNA expression of Ccl2, Cxcl1, Il10, Foxp3, Gzmb, Ifng, Arg1, Ido1, Il6, Il17, Nos2, Cd 274, Tgfb1, Tbx21 and Tnf in the whole lung normalized by Cd45 expression. (c) Relative mRNA expression of IκBα in the whole lung normalized to β-actin. (d) Western blot analysis of IκBα and β-actin protein levels in whole lung tissue. (e) Quantification of (d) relative density of IκBα/β-actin. Data represent mean ± SEM; **P < 0.01, *P < 0.05, experimental replicate #3.