Figure 3.

Gene-expression regulation by 1B3 in multiple cell lines

(A) Numbers of DE genes that are downregulated (estimated expression 1B3/estimated expression mock < 1) and upregulated (estimated expression 1B3/estimated expression mock > 1) 24 h and 72 h after transfection with 10 nM 1B3 in multiple (up to six) tumor cell lines are shown as compared to mock-transfection conditions. The number of downregulated genes that are predicted miR-193a-3p targets is also indicated. (B) Volcano plots show genes DE in at least three cell lines (blue) in the population of all DE genes in at least one cell line (black). (C) Venn diagram demonstrating DE genes common among at least three cell lines. Downward arrow indicates downregulation (average estimated expression 1B3/estimated expression mock < 1), and upward arrow indicates upregulation (average estimated expression 1B3/estimated expression mock > 1).