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Optimization of thread spray using the analysis of 100 μM aqueous protein solutions deposited on 100 % cotton. Positive-ion mode thread spray mass spectra for (A) cytochrome c (B) lysozyme and (C) cytochrome c at pH 3.0 and (D) myoglobin at pH 3.0. The spray voltage was 4 kV for all thread spray optimization experiments.
