Fig. 4.

In vitro transcription and translation of polycistronic loci. RNAs corresponding to polycistronic transcripts were synthesized from corresponding DNA templates (Methods) and translated in vitro in wheat germ extracts containing radiolabeled methionine (Met). The products were separated by denaturing polyacrylamide gel electrophoresis and visualized by fluorography. Upstream gene products are indicated by white triangles, and downstream gene products are indicated by black triangles. The polycistronic gene pairs and their expected sizes are presented as a table. Gene identifications from C. reinhardtii begin with “Cre,” and gene identifications from C. zofingiensis begin with “Cz.” The intensities of each band were normalized relative to the number of Met, and the ratios of the upstream to the downstream gene product for each pair are presented in the accompanying table.