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. 2021 Feb 8;118(7):e2015675118. doi: 10.1073/pnas.2015675118

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Fig. 2. — Cbt causes an ectopic cell cycle in pupal wings. (A and B) Thirty hours APF, wings with GFP⁺-marked clones (green) expressing cbt by hs-flp; tub > CD2 > Gal4;tub-Gal80^ts. Cbt induced ectopic DNA replication by EdU incorporation (red) and ectopic S and G2 phases by anti-CycA staining (red). Nuclei were stained with Hoechst (blue). (Scale bars, 20 μm.) (C, D, F, and G) FACS of ap-Gal4;tub-Gal80^ts wings with GFP labeled cbt expression (C and D) or cbt+stg coexpression (F and G) at 30 h APF (C and F) and 44 h APF (D and G). (E) Number of cells per clone for a given genotype, with clonal induction at 0 h APF.