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. 2021 Feb 8;118(7):e2004523118. doi: 10.1073/pnas.2004523118

Fig. 2.

Fig. 2.

Or35a ORNs mediating yeast odor-enhanced PER. (A) GC-MS analysis for yeast-derived volatile compounds. Volatile compounds are indicated in the order of abundance. (B) ORN screen for yeast odor-enhanced PER. The modified PER analysis was performed in flies expressing Kir2.1 in a subset of ORNs. n = 6 to 13. (C and D) Modified PER in Or35a or Orco mutant flies. The genotypes are indicated. n = 6 to 14. (E) Modified PER using volatile compounds found in yeast culture. n = 6. (F) PER upon optogenetic activation of Or35a ORNs. Or35a>ReaChR flies were tested in the absence or presence of all transretinal. n = 7 to 11. All data are presented as means ± SEM. Unpaired Student’s t tests or Mann–Whitney U tests were used for B–E. One-way ANOVAs with Tukey post hoc tests or Kruskal–Wallis with Mann–Whitney U tests were used for F. The asterisks indicate statistically significant differences from PER induced by sucrose alone, unless otherwise indicated. *P < 0.05, **P < 0.01, ***P < 0.001.