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. 2021 Feb 9;118(7):e2021342118. doi: 10.1073/pnas.2021342118

Fig. 4.

Fig. 4.

In vitro and in vivo treatment with rituximab (RTX) leads to CD20KO phenotype. (A) Flow cytometry analysis after treatment with RTX for 3 d showing the loss of CD19, IgM-BCR, and CD22 on Ramos-RTX compared to CD20KO-L, untreated Ramos-WT, and unstained control (Ctrl); n = 3 (B) Flow cytometry analysis of negatively selected naive B cells from peripheral blood of a healthy donor were treated with RTX for 60 min (HD-RTX) and compared to untreated (HD), to plasma cells (PCs) of the same donor, or left untreated and unstained (Ctrl); n = 3. (C) Example of B cells taken from EDTA whole-blood samples of RA patient undergoing RTX treatment after 0, 15, 30, 60, and 120 min. RTX (1 mg/mL) flow rate, 50 mL/h. Flow cytometry staining of CD19+/CD27−/IgM+ selected B cells shows internalization of IgM and CD19; n = 3.