Table 1.
Group | Medium | Treatment |
---|---|---|
Control | Osteogenic differentiation medium | No |
MO | Osteogenic differentiation medium + 10% Moringa leaf-containing serum | No |
OS + H2O2 | Osteogenic differentiation medium | 100 μmol/L H2O2 |
MO + H2O2 | Osteogenic differentiation medium + 10% Moringa leaf-containing serum | 100 μmol/L of H2O2 |
MO + H2O2 + Wor | Osteogenic differentiation medium + 10% Moringa leaf-containing serum | 100 μmol/L H2O2 and 0.5 μmol/L wortmannin |
MO + H2O2 + DMSO | Osteogenic differentiation medium + 10% Moringa leaf-containing serum | 100 μmol/L H2O2, 0.5 μmol/L of wortmannin and DMSO (its dosage was the same as the MO + H2O2 + Wor group) |
OS the third passage of BMSCs were cultured in osteogenic differentiation medium, MO the third passage of BMSCs were cultured in osteogenic differentiation medium + 10% Moringa leaf-containing serum, Wor wortmannin, an inhibitor of PI3K, DMSO solvent of wortmannin