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. 2021 Feb 20;21:107. doi: 10.1186/s12870-021-02875-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Sequential fluorescence in situ hybridization/genomic in situ hybridization (FISH/GISH) karyotype and chromosome plots of the cultivar Tifrunner. a FISH using Multiplex #3 (green) and Multiplex #4 (red); b GISH using genomic DNA of A. duranensis (green) and A. ipaensis (red); c FISH using 45S rDNA (red) and 5S rDNA (green) as the probes; d Karyotypes corresponding to chromosomes in the sequencing map of Tifrunner; SSON indicates the FISH karyotype using Multiplex #3 (green) and Multiplex #4 (red); GISH indicates the FISH karyotype using total genomic DNAs of A. duranensis (green) and A. ipaensis (red); 45S/5S indicates the FISH karyotype using 45S rDNA (red) and 5S rDNA (green); e Idiogram karyotype of Tifrunner; f chromosome plots of Tifrunner; blue arrows in (e) and (f) indicate the non-correspondent signals of oligos Ipa-1162 and Ipa-1137 between the karyotype and chromosome plots; green arrows in (e) and (f) indicate the non-correspondent signals of oligos TIF-439, TIF-185-1, TIF-134-3 and TIF-165-3 between the karyotype and chromosome plots; and red arrows in (e) and (f) indicate the non-correspondent signals of oligos DP-5 between the karyotype and chromosome plots. Scale bar: 10 μm