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. 2021 Feb 16;14:719–728. doi: 10.2147/DMSO.S288482

Figure 2.

Figure 2

IRS-2 is the potential target of miR-7a. (A) qRT-PCR analysis was done for studying the levels of miR-7a in endothelial cells and Retinal pericytes co-transfected with miR-7a mimic or mimic control.(B) qRT-PCR analysis in endometrial cells and Retinal pericytes co-transfected with miR-7a inhibitor or control.(C) The IRS-2 3ʹUTR having potential binding site for miR-7a. (D) The Endothelial cells co-transfected with miR-7a mimic or control luciferase vector having wild type or mutant IRS-2 3ʹUTR. (E) Results of luciferase assay in endothelial cells transfected with miR-7a inhibitor or control, the luciferase results were normalized against renilla luciferase. Results are presented as the mean ± standard deviation (n=3). *P<0.05 compared to other groups, @P<0.05 compared to miR-7a mimic control.