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. Author manuscript; available in PMC: 2021 Feb 20.
Published in final edited form as: Cell Rep. 2020 Dec 22;33(12):108543. doi: 10.1016/j.celrep.2020.108543

Figure 3. TREX2 Influences RFs in RAD51K133A Cells.

Figure 3.

(A) Fiber analysis to analyze RF stalling. TREX2 stalls RFs in HsRAD51K133A cells exposed to HU. Statistics: a chi-square test with Yates’ correction and Fisher’s exact test were performed using Prism8 software. At least 500 fibers were observed for each sample. For unexposed cells, all comparisons are p > 0.25.

(B) Fiber analysis of the impact of MRE11 and DNA2 on nascent strand degradation. Refer to legend for numerical identification of genotypes in (A). Red line: median for TREX2WT/RAD51KA. Statistics: Kruskal-Wallis test with Dunn’s multiple comparison test analyzed by Prism8 software. At least 100 fibers were observed for each sample.

(C) Dose response to camptothecin (CPT) on indicated cell lines assessed by colony-forming assay. Compared with the other genotypes, TREX2null/RAD51K133A cells exhibited enhanced sensitivity after exposure to CPT. Statistics: two-way ANOVA with Dunnett’s multiple comparisons test was performed using Prism8. Mean ± SD, n = 3. Statistics are represented by *p < 0.05, **p < 0.005, and ***p < 0.0005. NS, not significant.