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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Am J Reprod Immunol. 2020 Sep 21;85(3):e13343. doi: 10.1111/aji.13343

Figure 1.

Figure 1.

Representative strategy used to identify CD14+HLA-DRlo/- monocytic cell subsets in women with epithelial ovarian cancer. Mononuclear cells were analyzed using a series of sequential regions to exclude debris, apoptotic events, aggregates and CD45 negative events (not shown). Panel 1: A series of regions were created to exclude lineage positive (CD3, CD19, and CD56) cells (R1); Panel 2: CD33 and CD11b dual positive cells (R2); and Panel 3: HLADR negative to dim cells (R3). Panel 4: The CD15 vs. CD14 dotplot gated on R2, R3, and excluding cells in R1 was used to define CD14+HLA-DRlo/- monocytic cells (excludes cells in R4).