KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Human DNA-PKcs (full-length) | This paper | Residues 1-4128 |
| Human Ku70/80 | This paper | Residues 1-609 and 1-732 |
| Deposited Data | ||
| Re-refined apo-DNA-PKcs, X-ray (4.3 Å) | (Sibanda et al., 2017) | PDB: 7K17 |
| DNA-PKcs-DNA complex I, cryoEM (4.3 Å) | This paper | PDB: 7K19 EMDB: 22622 |
| DNA-PKcs-DNA complex II, cryoEM (4.3 Å) | This paper | PDB: 7K1B EMDB: 22623 |
| DNA-PK-DNA complex III, cryoEM (3.9 Å) | This paper | PDB: 7K1J EMDB: 22624 |
| DNA-PK-DNA complex IV, cryoEM (4.1 Å) | This paper | PDB: 7K1K EMDB: 22625 |
| DNA-PK-DNA complex V, cryoEM (3.9 Å) | This paper | PDB: 7K1N EMDB: 22626 |
| DNA-PK-DNA complex VI, cryoEM (3.7 Å) | This paper | PDB: 7K0Y EMDB: 22618 |
| Inactive FATKIN, cryoEM (3.2 Å) | This paper | PDB: 7K11 EMDB: 22620 |
| Active FATKIN, cryoEM (3.3 Å) | This paper | PDB: 7K10 EMDB: 22619 |
| Experimental Models: Cell Lines | ||
| HeLa-S3 cells | National Cell Culture Center | https://cellculturecompany.com/national-cell-culture-center/ |
| HEK293T cells | ATCC | CRL-3216 |
| Oligonucleotides | ||
| See DNA preparation in STAR Methods | This paper | N/A |
| Recombinant DNA | ||
| Ku70 in pLEXm plasmid | This paper | pWY2799 |
| Ku80 in pLEXm plasmid | This paper | pWY2802 |
| Software and Algorithms | ||
| PyMol | Schrodinger | https://pymol.org/2/ |
| Prism-8 | GraphPad Software | https://www.graphpad.com/ |
| CCP4 | CCP4 | http://www.ccp4.ac.uk |
| PHENIX | (Adams et al., 2010) | https://www.phenix-online.org |
| COOT | (Emsley, 2010) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| UCSF Chimera | (Pettersen et al., 2004) | https://www.cgl.ucsf.edu/chimera/ |
| cryoSPARC | (Punjani et al., 2017) | https://cryosparc.com/ |
| RELION | (Scheres, 2012); (Fernandez-Leiro and Scheres, 2017) | https://www2.mrc-lmb.cam.ac.uk/relion |
| SerialEM | (Mastronarde, 2005) | https://bio3d.colorado.edu/SerialEM/ |