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. Author manuscript; available in PMC: 2022 Feb 18.
Published in final edited form as: Mol Cell. 2020 Dec 21;81(4):859–869.e8. doi: 10.1016/j.molcel.2020.11.045

Figure 2 – Rescue of Tet2−/− iPSC reprogramming efficiency is dependent on TET2 fC/caC activity.

Figure 2 –

(A) Schematic illustration of Tet2−/− MEF reprogramming paradigm. (B) Quantification of AP staining of pluripotent colonies at day 10. Box plots indicate median AP+ colony forming units (CFUs) (one-way ANOVA with Tukey multiple comparisons; n=5; groups with different letters denote significant differences between groups, while shared letters indicate no difference was detected). (C) Quantification of immunohistochemical staining for NANOG-positive stable pluripotent colonies at day 18 (median NANOG+ CFU; one-way ANOVA with Tukey multiple comparisons; n=5). (D) Heat map of differentially expressed genes in Tet2-WT or Tet2-E transduced cells relative to vector control at day 5, with enriched GO terms indicated on right (RNA-seq; n=3–4; FDR < 0.05; fold-change > 1.5). (E) Venn overlap of significantly altered transcripts in Tet2-WT and Tet2-E transduced cells relative to vector control. (F) MA plot for Tet2-WT vs. Tet2-E gene expression. Red dots represent differentially expressed genes, with biologically relevant genes designated (FDR < 0.05; fold-change > 1.5).