Figure 4.

Plots showing how the Thon-ring modulation depths, in different resolution bands, decrease as the defocus is increased. Theoretical curves show the fraction of the area of the field-of-view for which both wavelets remain in the recorded image, whereas circles (9 Å), triangles (7 Å), and diamonds (2.3 Å) represent the experimental data shown in Figure 2B. The Thon-ring modulations at 2.3 Å come from the limited number of gold nanoparticles, some of which are not all in the correct orientation to diffract at that resolution, and others of which have fringes that are delocalized out of the field of view when the defocus is high enough. We believe that this may contribute to the scatter in the last few points at 2.3 Å.