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. Author manuscript; available in PMC: 2021 Feb 21.
Published in final edited form as: Cell Syst. 2020 Dec 15;12(2):141–158.e9. doi: 10.1016/j.cels.2020.11.007

Figure 1: Confirmation of Aβ42 levels and amyloid pathology in AppNL-F/NL-F, and AppNL-G-F/NL-G-F mouse brains.

Figure 1:

(A) Aβ42 levels in cortical homogenates (GuHCl soluble) from AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 2, 4, 6, 8, 10, and 12 months of age as measured by sandwich ELISA with analytical sensitivity range of 15.6 – 1,000 pg / mL. (B) Aβ42 levels in cortical homogenates (GuHCl soluble) from AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 4, 6, and 8 months of age as measured by an ultrasensitive sandwich ELISA with analytical sensitivity range of 1.56 – 100 pg / mL. (C) Oligomeric Aβ42 levels in TBST soluble cortical extracts from AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 4, 6, and 8 months of age as measured by a sandwich ELISA using the oligomeric preferential MOAB-2 antibody. (D) Amyloid pathology in App KI cortex and hippocampus. Representative thioflavin S stained sagittal brain sections from 6- and 12-month-old AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice. Scale bar = 500 μm. (E) Quantification of amyloid plaque area from panel (D). Data represents average thioflavin S positive stained area relative to the total cortical or hippocampal area per section. (F) Amyloid pathology in App KI cerebellum. Representative brain sections from 6- and 12-month-old AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice stained with thioflavin S. Scale bar = 500 μm. (G) Quantification of amyloid plaque area from panel (F) Data represents average thioflavin S positive stained area relative to the total cerebellar area per section. (H) Dot blot analysis of Aβ (82E1), amyloid fibrils (LOC), and actin using aggregated protein fractions from cortical extracts of AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 6 months of age. (I) Quantification of Aβ (82E1) and amyloid fibrils (LOC) levels from panel (H). (A) N = 3 – 8 mice of mixed gender, (B-C) N = 4 mice of mixed gender, (E, G, I) N = 3 mice of mixed gender, per group. Each datapoint was an average of 2 – 3 30 μm sections per mouse. All data represent mean ± SD, analyzed by one-way ANOVA followed by Fisher’s LSD. Circles represent individual biological replicates. * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001.