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. Author manuscript; available in PMC: 2021 Feb 21.
Published in final edited form as: Cell Syst. 2020 Dec 15;12(2):141–158.e9. doi: 10.1016/j.cels.2020.11.007

Figure 7: AppNL-G-F/NL-G-F hippocampus has an enlarged SV pool and enhanced post-tetanic potentiation.

Figure 7:

(A-C) Representative electron tomography sections of individual synapses from App KI CA1 at six months of age. (D-F) Reconstructed 3-D models of the axon terminals from adjacent tomograms (100–150 planes per model). Plasma membrane is modeled in green; SVs are in blue; the PSD is in yellow, and large irregular structures are in purple. (G) Quantification of SV density (SV / nm3) in the hippocampal CA1 region (top) and cortex (bottom). AppNL-G-F/NL-G-F has greater SV density in CA1 and cortex, and AppNL-F/NL-F has greater density in CA1 compared to AppNL/NL. Each circle represents an individual synapse and the number of vesicles in that synapse. N = 3 mice and 3–7 synapses per mouse. Data represent mean ± SEM, analyzed with one-way ANOVA followed by Fisher’s LSD. (H) Representative traces of paired fEPSPs in AppNL-G-F/NL-G-F or AppNL/NL CA1 pyramidal cells, following stimulation of Schaffer collateral with increasing intensity. (I) Quantification showing no difference in field responses across stimulation intensities in AppNL-G-F/NL-G-F compared to AppNL/NL. (J) Representative traces from sequential stimulations across the indicated period. (K) Quantification showing no difference in paired stimulation ratio in AppNL-G-F/NL-G-F compared to AppNL/NL. (L) Plot representing cumulative fEPSPs (EPSP1 + EPSP2 + … + EPSP1200) in response to a train of stimulation (1200 at 20 Hz) in AppNL-G-F/NL-G-F and AppNL/NL slices. The average cumulative fEPSP is significantly elevated in AppNL-G-F/NL-G-F compared to AppNL/NL. (M) The number of functional SVs (N*q) is elevated in AppNL-G-F/NL-G-F CA1 compared to AppNL/NL. (N) mEPSC amplitude was not significantly different in AppNL-G-F/NL-G-F compared to AppNL/NL. (O) Potentiation following post theta burst stimulation (TBS) is significantly increased in AppNL-G-F/NL-G-F compared to AppNL/NL. (M-O) Data represents mean ± SEM, analyzed with Student’s t-test. * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001. (A-F) Scale bar = 100 nm.