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. 2020 Dec 15;70(3):720–732. doi: 10.2337/db20-0634

Figure 7.

Figure 7

Reducing eosinophils does not affect insulin sensitivity in HFD-fed cKO mice. Male cKO mice that had been fed HFD for 12 weeks were injected intraperitoneally with 40 μg of anti-mouse IL-5 antibody (Ab) or isotype control once every 72 h for a total of 11 injections. Eosinophil content in pAT and iAT analyzed using flow cytometry at 2 days after the 11th injection (A). ITT performed at 2 days after the ninth injection (B). n = 4 mice/group. GTT performed at 2 days after the 10th injection (C). n = 4 mice/group. Macrophages and DCs (D) and T cells (E) analyzed using flow cytometry and mRNA levels of UCP1 and Prdm16 examined by RT-qPCR (F) in AT at 2 days after the 11th injection. *P < 0.05, ***P < 0.001.